Indigo Carmine, Certified, C.I. #73015

(Acid Blue 74; Carmine Blue; Indigotine 1, 1A)
C16H8N2O8S2Na2   F.W.466.36   CAS #860-22-0
Dye content: approx. 85%
Solubility: 1.3% Water; 0.0% Alcohol; 4.0% Cellosolve; 2.85% Glycol; 0.0% Xylene
Shunway's stain for animal embryos. Used with Acid Fuchsin for staining Negri bodies. Stain Tech., 5, 34 (1928)

Ingrain Blue, C.I. #74240 ( Alcian Blue 8GX )

Isopropyl Alcohol, Reagent, A.C.S.

(2-Propanol)
CH3CH(OH)CH3   F.W. 60.10   CAS #67-63-0
A.C.S. Specifications:

Color (APHA)

.

10

Density @ 25°C

.

0.781-0.783

Boiling Range (including 82.3°C)

.

1°C

Titratable Acid or Base

.

0.0001 meq/g

Residue after Evaporation

.

0.001%

Solubility in water

.

To pass test

Water

.

0.2%

 

Lanthanum Nitrate, Hexahydrate, Reagent

La(NO3)3·6H20   F.W. 433.03   CAS #10277-43-7

m.p.

..

40°C

Assay

..

Min 99.9%

Calcium

..

0.001%

Magnesium

..

0.0001%

A metal stain, intracellular tracer.

 

Lead Acetate, Trihydrate Reagent, A.C.S.

Pb(CH3COO)2·3H20   F.W. 379.33   CAS #6080-56-4
A.C.S. Specifications:

Assay

.

99.0-103.0%

Chloride

.

5ppm

Copper

.

0.002%

Insoluble Matter

.

 0.01%

Iron

.

0.001%

Nitrate and Nitrite (as NO3)

.

To pass test

 

 

Lead Citrate, Trihydrate

Pb(C6H5O7)2·3H2O   FW 1053.82   CAS #512-26-5
Minimum assay 99.%

Lead Nitrate, Reagent, A.C.S.

Pb(NO3)2   F.W. 331.21   CAS #10099-74-8

Minimum Assay

 

99.5%

Insoluble matter

 

0.005%

Reaction

 

Not less than pH 3.5

Chloride

 

0.0005%

Calcium

 

0.01%

Copper

 

0.0005%

Iron

 

0.0005%

Potassium

 

0.002%

Sodium.

 

0.002%

Light Green SF Yellowish, Certified, C.I. #42095

(Acid Green 5; Light Green 2G, S or 2GN; Acid Green F; Fast Acid Green N)
C37H34N2Na2O9S3   F.W. 792.86   CAS #5141-20-8
Solubility: 20.35% Water; 4.0% Alcohol; 5.25% Cellosolve; 12.0% Glycol; 0.0% Xylene

Lucifer Yellow, CH, Dilithium Salt

C13H9Li2N2O9S2   F.W. 457.25   CAS #67769-47-5

A technique producing an electron dense osmiophilic polymer when Lucifer Yellow is injected into cells. Lucifer Yellow also can be viewed in living cells as a dye marker and is more sensitive than any other dye.

Magnesium Chloride, Hexahydrate, Reagent, A.C.S. MgCl2·6H2O   F.W. 203.30  CAS #7791-18-6  Assay: 99.0-102.0%

 

Malachite Green 4, Certified ( Basic Green Oxalate )

Maleic Acid, Crystalline, Repurified, A.C.S.

(Toxilic Acid; cis-Butenediodic Acid)
HOOCCH:CHCOOH   F.W. 116.08   CAS #110-16-7
m.p. 132-134°C
Assay 99.5%

Insoluble Matter

 

0.005%

Fumaric Acid

 

0.15%

Chloride

 

0.01%

Sulfate.

 

0.001%

 

Melamine

(Sym Triaminotriazine)
C3H6N6   F.W. 126.12   CAS #108-78-1
m.p. 301°C
The melamine mixture has been used for embedding nerve tissue (Shinagawa and Shinagawa, 1978). Also, Melamine-Formaldehyde can be modified into a water-soluble embedding medium.

Methacrylic Acid, Glacial

(2-Methacrylic Acid)
CH2:C(CH3)COOH   F.W. 86.09   CAS #79-41-4
Assay: >98%
Stab. with 0.25% Hydroquinone monomethyl ether

Methanol, Reagent, A.C.S.

(Methyl Alcohol)
CH3OH   F.W. 32.04   CAS #67-56-1
Assay: 99.8%
Specific Gravity : 0.79
A.C.S. Specifications:

Residue after Evaporation

.

0.001%

Boiling Range (Boiling point 64.6°C)

.

2°C max

Appearance

.

Clear

Color (A.P.H.A.)

.

10 max

Acetone & Aldehydes (0.001%)

.

To pass test

Titratable Acid

.

0.0003 meq/g

Titratable Base

.

0.0002 meq/g

Water

.

0.2% max

 

2-Methyl Butane, Reagent (Isopropentane)
CH2H5CH(CH3)2   F.W. 72.15   CAS #78-78-4
b.p. 27-31°C
Specific Gravity @ 20°C: 0.62

Methyl Cellulose, 400 cPS,USP/NF

(Methylcel MC; Cellulose Methyl Ether)
CAS #9004-67-5
Methoxyl (-CH3) content : 27.5-31.5%
Medium viscosity (2% in water, @20°C): 230-550 cps
Used with polyethylene glycol in the presence of acidic uranyl acetate to stain cryo sections.

Methylene Blue, Certified, C.I. #52015

(Basic Blue 9; Swiss Blue)
C16H18N3SCl   F.W. 319.86+aq   CAS #7220-79-3
Solubility: 9.5% Water; 6.0% Alcohol; 6.5% Cellosolve; 10.0% Glycol; 0.0% Xylene.
* Used to distinguish autonomic nerves. Richardson, K.C., (1969) Anat. Rec., 164, 231.

Methyl Ethyl Ketone, Reagent

(2-Butanone)
CH3COCH2CH3   F.W. 72.11   CAS #78-93-3

Assay

 

>98%

Specific Gravity (H2O=1) :

 

0.799-0.802

Acidity (as CH3COOH)

 

0.003%

Water

 

0.2%

 

Methylene Chloride, Reagent, A.C.S.

(Dichloromethane; Plastisolve)
CH2Cl2   F.W. 84.93   CAS #75-09-2

Methyl Green, Certified, C.I. #42590

(Zinc Chloride Salt; Ethyl Green)
C27H35N3C12·ZnC12   F.W. 608.79   CAS #7114-03-6
Solubility: 8.0% water; 3.0% Alcohol; 1.8% Cellosolve; 3.5% Glycol; 0.0% Xylene.
* Used as a counterstain in Almann's aniline acid fuchsin technique for mitochrondria; used in Kurnick's Methyl Green-Pyonin stain for nuclei and cystoplasmic granules of liver cells. Marker for DNA at acid and neutral conditions. J.B. Boyd, H.R. Mitchell, (1965) Anal. Biochem., 13, 28.

Methyl Methacrylate, Monomer

(Methacrylic Acid Methyl Ester)
CH2·C(CH3)COOCH3   F.W. 100.12   CAS #80-62-6
b.p. 99-100°C
Specific Gravity @20°C : 0.943
Inhibitor (Hydroquinone): 0.0025% (25ppm)

Methyl Violet 2B, Certified, C.I. #42535

(Basic Violet 1; Dahlia B; Gentian Violet; Paris Violet; Pyoktanium Coeruleum)
C24H28N4Cl   F.W. 393.96   CAS #8004-87-3
Solubility: 9.0% Water; 8.25% Alcohol; 9.0% Cellosolve; 8.5% Glycol;0.01% Xylene
Dye content: 75% min
Used with Methyl Green to stain Epoxy-embedded sections.